Jon's Blog
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Photo-Morpholinos activated in zebrafish tails
Photocleavable Morpholino oligos were used to disrupt Piezo1 function in zebrafish embryos; antisense activity was released in the tails of zebrafish with a steerable UV light beam.
Crowding induces live cell extrusion to maintain homeostatic cell numbers in epithelia. Eisenhoffer GT, Loftus PD, Yoshigi M, Otsuna H, Chien CB, Morcos PA, Rosenblatt J. Nature. 2012 Apr 15;484(7395):546-9. doi: 10.1038/nature10999. -
Morpholino probes for in situ hybridization
Anne has shown that fluoresceinated Morpholinos, in combination with antibodies binding fluorescein, are effective as in situ hybridization probes in zebrafish embryos.
Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues. Lagendijk AK, Moulton JD, Bakkers J. Biology Open. 2012;[Advance Online Publication] doi:10.1242/bio.2012810
http://bio.biologists.org/content/early/2012/04/11/bio.2012810.full
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Turning gene function ON and OFF using sense and antisense photo-morpholinos in zebrafish
Tallafuss A, Gibson D, Morcos P, Li Y, Seredick S, Eisen J, Washbourne P. Turning gene function ON and OFF using sense and antisense photo-morpholinos in zebrafish. Development. 2012;139:1691-1699. doi:10.1242/dev.072702 -
Cell-penetrating peptides enhance systemic delivery of antisense morpholino oligomers
Moulton HM. Cell-penetrating peptides enhance systemic delivery of antisense morpholino oligomers. Methods Mol Biol. 2012;867:407-14.
http://www.ncbi.nlm.nih.gov/pubmed/22454076
My sweetheart's work :D