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We have identified mutant strains of S. cerevisiae



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Member Since5/4/2006
Band MembersAlesio Antonini, Experience. S. cerevisiae strains containing RAS2val19, a RAS2 gene with a missense mutation analogous to one that activates the transforming potential of mammalian ras genes, have growth and biochemical properties strikingly similar to yeast strains carrying IAC or bcy1. Yeast strains carrying the IAC mutation have elevated levels of adenylate cyclase activity. bcy1 is a mutation that suppresses the lethality in adenylate cyclase deficient yeast. Yeast strains deficient in RAS function exhibit properties similar to adenylate cyclase deficient yeast. bcy1 suppresses lethality in ras1-ras2- yeast. Compared to wild-type yeast strains, intracellular cyclic AMP levels are significantly elevated in RAS2val19 strains, significantly depressed in ras2- strains, and virtually undetectable in ras1-ras2-bcy1 strains. Membranes from ras1-ras2-bcy1 yeast lack the GTP-stimulated adenylate cyclase activity present in membranes from wild-type cells, and membranes from RAS2val19 yeast strains have elevated levels of an apparently GTP-independent adenylate cyclase activity. Mixing membranes from ras1-ras2- yeast with membranes from adenylate cyclase deficient yeast reconstitutes a GTP-dependent adenylate cyclase.An important recent advance in the functional analysis of Saccharomyces cerevisiae genes is the development of the one-step PCR-mediated technique for deletion and modification of chromosomal genes. This method allows very rapid gene manipulations without requiring plasmid clones of the gene of interest. We describe here a new set of plasmids that serve as templates for the PCR synthesis of fragments that allow a variety of gene modifications. Using as selectable marker the S. cerevisiae TRP1 gene or modules containing the heterologous Schizosaccharomyces pombe his5+ or Escherichia coli kanr gene, these plasmids allow gene deletion, gene over..using the regulatable GAL1 promoter), C- or N-terminal protein tagging [with GFP(S65T), GST, or the 3HA or 13Myc epitope], and partial N- or C-terminal deletions (with or without concomitant protein tagging). Because of the modular nature of the plasmids, they allow efficient and economical use of a small number of PCR primers for a wide variety of gene manipulations. Thus, these plasmids should further facilitate the rapid analysis of gene function in S. cerevisiae.
InfluencesMonowatt,Dilatazione,sfingolipide,tetranicus urticae,Yatanawal Nuri Buzid fi hadhihi al-maqalah al-mutarjamah ila al-Injiliziyah [al-sadirah fi al-tariq, al-sinah 47, al-'adad 4 (Aylul Sibtimbir 1988)] mawdu' al-waqi'iyah al-jadidah fi al-sinima al-'Arabiyah. Wa-allati yad'u ilayha ka-badil lima huwa rahin. Wa-fima yali muqtatifat min al-maqalah allati turjimat bil-kamil ila al-injiliziyah fi hadha al-'adad: "Rubbama la takfi kalimat "hazimah" lil-ta'bir 'an wad' al-'alam al-'Arabi, li-anna al-azmah allati yatakhabbat fiha hiya a'maq min an takun murtabitah bi-hazimat 1967 li-annaha laysat azmah zarifah aw mutamarkizah fi balad dun al-akhar bi-qadr ma hiya azmah tarikhiyah, haykaliyah, mutajadhdhirah wa-tarja' usuluha ila al-inhitat al-hadari al-ladhi 'ashathu al-buldan al-'Arabiyah al-Islamiyah mundhu qurun 'adidah." "Tu'tabar aflam "al-Mumya'",saccharomyces cerevisiae "al-Mukhdu'un" wa "al-'Asfur" thalathah ibda'at mujaddidah tamaman fi madmuniha wa-shakliha... "al-Mumya'" yabda' tajribah ra'idah fi mawdu' asbaha simmah min simmat al-waqi'yah al-jadidah wa-huwa al-bahth 'an "al-hawiyah" wa-al-'ilaqah bil-turath wa bil-shakhsiyah... wa-ya'ti "al-Mukhdu'un" tribolium castaneum li-tawfiq Salih ka-tatwij li-a'mal al-mukhrij al-sabiqah allati hawala fiha al-qat' ma'a al-miludrama wa-lakin lam yakun al-qat' niha'iyan illa fi "al-Mukhdu'un". Fa-huwa yuqaddim khafaya al-qadiyah al-Filastiniyah wa al-wajh al-'Arabi li-taqtil al-Filastiniyyin... Amma film "al-'Usfur" fa-huwa qimmah al-sinima al-'Arabiyah 'ala al-itlaq. Jadid fi mawdu'uhi 'ala al-mustawa al-'alami... Hadhihi al-aflam al-thalathah tajtami' fi niqtah hammah: Inna al-insan al-basit makhdu' min taraf al-sultah, wa-hazimatahu tukmin fi annahu sallama al-sultah li-ghayrih, wa-qabl al-wasayah." "Amma al-jil al-jadid min al-sinima'iyin al-ladhina wasalu masirat Tawfiq Salah wa Shahin wa Shadi 'Abd al-Salam fahum fi aghlabihim, in lam yakunu kullahum, min kharij Misr. Wa-bil-tahdid min buldan al-Maghrib al-'Arabi wa Suriyya wa Lubnan cazo in culo wa Falistin wa-aghlabahum min mawalid al-arba'inat". "Wa-min khususiyat al-waqi'iyah al-jadidah al-bahth 'an al-hawiyah allati asbahat 'inda al-'adid niqtat istifham. Wa al-bahth 'an al-hawiyah ya'ni al-bahth 'an al-shakhsiyah wa al-bahth 'an al-madi al-majid al-muzdahir amilan fi irja'ihi... [wa-aydan] min khususiyat al-waqi'iyah al-jadidah tahtim al-akadhib wa al-usturah allati banaha al-Adab al-qadim wa al-sinima al-qadimah". "Wa-idha aradna talkhis al-waqi'iyah al-jadidah fi jumlah naqul: "Khalq al-jadid ma'a al-jamil". Laysat hunak namadhij wa-la kalishihat tataba', bal tatahaddad al-mawadi' wa al-turuq bi-ta'addud al-mukhrijin." "al-Sinima al-jadidah mushattatah, muba'tharah, aghlabaha dafin al-sanadiq wa al-'ulab, al-kullu yarid an yata'arraf 'alayha bidun an yashtariha, kullu sinah tazdad su'ubah tamwilaha, hatta al-i'anat allati kana yahsal 'alayha al-mukhrijun min Urubba taqallasat... al-hal fi aydina wa-laysa fi ayyi namudhaj wa-la itijah."
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   About alesio antonini experience
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Alesio Antonini is a project born in Italy in 1994.
During this week Alesio Antonini Experience
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    trhqrwhttrhrrttr We have identified mutant strains of S. cerevisiae that fail to properly arrest their cell cycles at mitosis in response to the loss of microtubule function. New bud emergence and DNA replication (but not cytokinesis) occur with high efficiency in the mutants under conditions that inhibit these events in wild-type cells. The inability to halt cell cycle progression is specific for impaired microtubule function; the mutants respond normally to other cell cycle-blocking treatments. Under microtubule-disrupting conditions, the mutants neither achieve nor maintain the high level of histone H1 kinase activity characteristic of wild-type cells. Our studies have defined three genes required for normal cell cycle arrest. These findings are consistent with the existence of a surveillance system that halts the cell cycle in response to microtubule perturbation.We have identified mutant strains of S. cerevisiae that fail to properly arrest their cell cycles at mitosis in response to the loss of microtubule function. New bud emergence and DNA replication (but not cytokinesis) occur with high efficiency in the mutants under conditions that inhibit these events in wild-type cells. The inability to halt cell cycle progression is specific for impaired microtubule function; the mutants respond normally to other cell cycle-blocking treatments. Under microtubule-disrupting conditions, the mutants neither achieve nor maintain the high level of histone H1 kinase activity characteristic of wild-type cells. Our studies have defined three genes required for normal cell cycle arrest. These findings are consistent with the existence of a surveillance system that halts the cell cycle in response to microtubule perturbation.
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    cellpadding='px'>? ?:?????DNA????????,????????????????????????????????????????,???????44,67,85 ku???,?????????????16.82%,15.62%,?????42.94%,??????????????,????,?????????????DNA??????????????????????????????,???????????????? ???:??;???DNA;??DNA??;???;??? ???:Q343.1+5 ?????:A ????:1000-2782(2000)01-0006-05 An analysis of variation characteristics of the progenies derived from introducing leymus racemosus gene into common wheat ZHU Xin-chan LIAO Xiang-ru FU Zheng-guang (Laboratory of Plant Molecularly Biology,Northwestern Agricultural University,Yangling,Shaanxi 712100,China) Abstract:Exogenous DNA directly introduced into wheat plant induced striking change in seed protein components of wheat and isozymogram of peroxidase isozyme (POD),esterase isozyme (EST) and superoxide dismutase (SOD).The proteins increased by 44 ku,67 ku,85 ku new compoents,and the protein and amino acid contents enhanced by 16.85% and 15.62% separately,Lys increased by 42.94%.Isozyme also showed difference isozyme bands,losing isozyme bands and increase and decrease of enzyme activity in varying degrees.Some bio-characteristics of variant grains derived from introducing exogenous DNA into common wheat,such as colour,hardness,endosperm trasparency etc,also showed remarkable changes.These variations can be stably expressed in successive progenies. Key words:wheat;leymus recemosus gene;exogenous DNA introduction;protein;isozyme? ?????????????,???????????,????????????????????[1,2]???????????????????????????????????,?????1A,1B,1D????3???????????????????,?????????????[3,4]?????????????????,????????????,??????????70????????×??[5]???×??[6]???×??[7]??????????????[8~11]?????????????????,?????????????????,???????????,????????????????????????????DNA??????,??????????????? 1 ????? 1.1 ? ? ???(Leymus racemosus),?????????????????,????(Triticum aestivum)?????,???????????????? 1.2 ? ? 1.2.1 DNA?? ??????????,????????,??????-????????DNA,???UV-120?????,A260/A280=1.893,A260/A230=2.173,??????????,?????DNA???????????[12,13]? 1.2.2 ??DNA?? ?????????,????5?,??????,??????????DNA 10 µL(4~5 g/L),??,????,????????,???????,??????????? 1.2.3 ???????SDS-PAGE ??????[14]???? 1.2.4 ????? ???Vs-KTP?????,?????????? 1.2.5 ??????? ????121-MB????????????????? 1.2.6 ???PAGE?? ?????[15]???,??????????0.5 g,????3 mL Tris-HCl(pH 8.0)???,?????,0~4???,????????,??????20 µL??????????Cs-930???????????,??????????????????370?525 nm,?????????????,??600 nm? 2 ????? 2.1 ??????????? ?1??,????DNA????????????????????,????????,????M1???85 ku????; M2????44, 67 ku????,36~67 ku??????????????(???),????,???????,?????????????????????????????,????????????? t07-1.gif (19069 ??) ?1 ????????SDS-PAGE P.??(?????);CK.??; M1,M2.????,???? 2.2 ??????????????? ??1??,????DNA??????????????????????,????M2????,???????????????????16.82%,42.94%?15.62%,?????????????? ?1 ?????????????????? mg.g-1 ?? ??? ??? ???? CK 141.5 3.40 118.75 M1 150.1 3.69 125.11 ? 8.6 0.29 6.36 M2 165.3 4.86 137.30 ? 23.8 1.46 18.55 ?:CK???;M1,M2?????;?????? 2.3 ??????? ?2??,????DNA?????????????????????????,?????????(POD)???????????,???M2????,??????4?,??????2.147?,??????????;?????(EST),M1??????3?,??????1.66?,?M2????????????????;???????(SOD),M1??????????,?M2??????2???,???3?????,????EST???? t08-1.gif (34762 ??) ?2 ???????????(PAGE) 2.4 ???????? ????DNA??????????????????,?M1????????????,?M2????????????,????????????,????????????????? 3 ? ? ??????????????,?????,???????,??????????????????????????[16,17]?????????????????,??????,DNA????,??DNA?????????DNA????????,???????,??????????????,????????????,???????DNA????????????????,?????? ?????????????????,???????????DNA??,??????????????????????,???????????????????????,???????????,??????????????[18],??????????????????,?????DNA????,???????????????,?????????????,????????,??????????????????????,??DNA??????????????????????,?????????(????????)? ???????????,??,???????????????????????????,???????????????????DNA?????????????POD,EST?SOD????????,???????????????????,???????DNA????????????????????????? ??DNA?????????,???????,?????????????????,????????????DNA?????????????,????????,???????????,??????????,????,???????????????????,????????????¦ ????:?????????????(99SM05) ????:???(1959-),?,?? ????:???(??????????????,???? 712100) ???(??????????????,???? 712100) ???(??????????????,???? 712100) ????: ?????DNA???RAPD?????? DNA extraction and RAPD reaction system of tea plant[Camellia sinensis(L.) O.Kuntze] <>2001?01? ?? , ??? , ??? , ??? , ??? DNA ????????RAPD????ir cell cycles at mitosis in response to the loss of microtubule function. New bud emergence and DNA replication (but not cytokinesis) occur with high efficiency in the mutants under conditions that inhibit these events in wild-type cells. The inability to halt cell cycle progression is specific for impaired microtubule function; the mutants respond normally to other cell cycle-blocking treatments. Under microtubule-disrupting conditions, the mutants neither achieve nor maintain the high level of histone H1 kinase activity characteristic of wild-type cells. Our studies have defined three genes required for normal cell cycle arrest. These findings are consistent with the existence of a surveillance system that halts the cell cycle in response to microtubule perturbation.We have identified mutant strains of S. cerevisiae that fail to properly arrest their cell cycles at mitosis in response to the loss of microtubule function. New bud emergence and DNA replication (but not cytokinesis) occur with high efficiency in the mutants under conditions that inhibit these events in wild-type cells. The inability to halt cell cycle progression is specific for impaired microtubule function; the mutants respond normally to other cell cycle-blocking treatments. Under microtubule-disrupting conditions, the mutants neither achieve nor maintain the high level of histone H1 kinase activity characteristic of wild-type cells. Our studies have defined three genes required for normal cell cycle arrest. These findings are consistent with the existence of a surveillance system that halts the cell cycle in response to microtubule
    perturbation.??????????????????????????,?????????????????,??????????????????????,???????DNA???????——SDS?????????????????????DNA,????DNA????? RAPD????????RAPD???????????????,?????RAPD???????,??25ul?????,?20-50ng?? DNA,1.5mmol/lMgCl2,?0.1mmol/lDNTPs,0.2umol/L???1UTaqDNA???;?????:95???? 300s,94???60s,35???60s,72???120s,???40???,??72???600s,?????????????? The success of RAPD reactions are decided by the qual ity of DNAand extraction method.For the tea shoot containing lots of polyphen ols and proteins in the cytoplasm which should be removed before the nucleus wa s decomposed by modified micro SDS method,Purified DNA were obtained from several tea variet ies, by which RAPD reaction were conducted.The better RAPD reaction system were structured by studying the factors affecting the tea RAPD reaction. The system was 20-25ng tea DNA,1.5mmol/L MgCl2,0.1mmol/LdNTPs for each nucleotide,0.2µ mo l/L primer and 1U Taq DNA polymerase in 25µl.The process of reaction was 95? f o r 300s in the beginning,then 94? for 60s,35? for 60s,72? for 120s,for 40 cyc les,72?for 600s in the end,good RAPD could be obtained. hwrmghkmklgpokhpkrohpokrwhpokrhopkrphokokthopkrtopkrptokrpkotpokrtopkrpktpkroòkgòklgltòktpkoptokpr
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    ' width='px' height='px' border='0px' /> We have identified mutant strains of S. cerevisiae that fail to properly arrest their cell cycles at mitosis in response to the loss of microtubule function. New bud emergence and DNA replication (but not cytokinesis) occur with high efficiency in the mutants under conditions that inhibit these events in wild-type cells. The inability to halt cell cycle progression is specific for impaired microtubule function; the mutants respond normally to other cell cycle-blocking treatments. Under microtubule-disrupting conditions, the mutants neither achieve nor maintain the high level of histone H1 kinase activity characteristic of wild-type cells. Our studies have defined three genes required for normal cell cycle arrest. These findings are consistent with the existence of a surveillance system that halts the cell cycle in response to microtubule perturbation.We have identified mutant strains of S. cerevisiae that fail to properly arrest their cell cycles at mitosis in response to the loss of microtubule function. New bud emergence and DNA replication (but not cytokinesis) occur with high efficiency in the mutants under conditions that inhibit these events in wild-type cells. The inability to halt cell cycle progression is specific for impaired microtubule function; the mutants respond normally to other cell cycle-blocking treatments. Under microtubule-disrupting conditions, the mutants neither achieve nor maintain the high level of histone H1 kinase activity characteristic of wild-type cells. Our studies have defined three genes required for normal cell cycle arrest. These findings are consistent with the existence of a surveillance system that halts the cell cycle in response to microtubule perturbation.
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    gWe have identified mutant strains of S. cerevisiae that fail to properly arrest their cell cycles at mitosis in response to the loss of microtubule function. New bud emergence and DNA replication (but not cytokinesis) occur with high efficiency in the mutants under conditions that inhibit these events in wild-type cells. The inability to halt cell cycle progression is specific for impaired microtubule function; the mutants respond normally to other cell cycle-blocking treatments. Under microtubule-disrupting conditions, the mutants neither achieve nor maintain the high level of histone H1 kinase activity characteristic of wild-type cells. Our studies have defined three genes required for normal cell cycle arrest. These findings are consistent with the existence of a surveillance system that halts the cell cycle in response to microtubule perturbation.We have identified mutant strains of S. cerevisiae that fail to properly arrest their cell cycles at mitosis in response to the loss of microtubule function. New bud emergence and DNA replication (but not cytokinesis) occur with high efficiency in the mutants under conditions that inhibit these events in wild-type cells. The inability to halt cell cycle progression is specific for impaired microtubule function; the mutants respond normally to other cell cycle-blocking treatments. Under microtubule-disrupting conditions, the mutants neither achieve nor maintain the high level of histone H1 kinase activity characteristic of wild-type cells. Our studies have defined three genes required for normal cell cycle arrest. These findings are consistent with the existence of a surveillance system that halts the cell cycle in response to microtubule perturbation.
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    alesio antonini experience's Friends Comments
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    DRESS:CODE Discoteka

    DRESS:CODE ex Insomnia



    Jan 6 2010 1:50 AM

    Ti informiamo che il Dress è in FERIE sino all'8 Gennaio....... Dall'8 Gennaio la sala Rock torna in SALA CENTRALE/Divine Stage e la 60s/Vintage torna nella Sala PENELOPE .... Il nuovo gestore, Niccolò Cretella, vi aspetta per del sano e fottuto Rock'n'Roll! Come quello che ha prodotto nel mitico Rolling Stone di Milano, di cui ne aveva le "redini" dal 1986 ---- Il Rolling era il tempio del Rock italiano, ora, dopo la chiusura avvenuta nel maggio scorso, il "testimone" verrà passato al Dress! ---- PROGRAMMA VENERDI 8 GENNAIO Dalle 23.30 alle 00.30 Live Music con: - 
 ALCOLICA (Tribute Band Metallica) www.myspace.com/alcolicabandpisa - 
 da mezzanotte 4 AREE danzanti: --- Rock 360° Area in Divine Stage: ROLLING STONE NIGHT con Marco Garavelli e Max de Riu + RockVJset con 500 Miles ---- Vintage Area (dagli anni ’50 ai ’90) nel Penelope: CAPTAIN Zerbo + MixClassicMovies ---- Electro’n’Roll Area: Luca di Venere + NU Combo Visuals ---- Jumparty Area (Hip-Hop /R’n’B/Funky): DJ Evo ---- RICORDA su www.dresscodeweb.com scarichi gratuitamente la RockboardCard, mostrala all'ingresso per ricevere la riduzione --- OMAGGIO UOMO FINO ALLE 23.30 --- DONNE OMAGGIO FINO ALLE 2.00 --- Dalle 23.31 INTERO 12 Euro , RIDOTTO (con RockBoardCard) 10 Euro ------------------------- FACEBOOK: Dress Code Disko
    DRESS:CODE Discoteka

    DRESS:CODE ex Insomnia



    Aug 30 2009 1:30 PM

    Ciao!! Venerdì 11 Settembre riapre il Dress:Code con le RockboardNight, anche quest'anno, come ogni anno, proporrà delle novità nelle programmazioni settimanali. Nella mitica PatchworkPlace, che ha visto passare in più di 20 anni i migliori djs e migliaia di clubbers saltare nel suo dancefloor, da quest'anno per TUTTI I VENERDI', proponiamo un genere che sta invadendo l'Europa: l'ELECTRO, che è di fatto musica elettronica contaminata da strumenti e voci rockstyle; all'interno del panorama electro ci sono due sub realtà, quella Pop (sullo stile di Tiga, Justice, ecc...) e quella più Techno (sullo stile di Steve Aoki, Erol Alkan, ecc..). Ti consigliamo di intervenire con lo spirito giusto come dice il motto di Electro'n'Roll: Sex, Fun and Electro'n'Roll, dove appunto è garantita un'ottima programmazione Electro da far Rollare tutti i presenti in pista, che devono essere ben predisposti, mentalmente, per una serata Polixesual, nonchè ben agghindati e molto appariscenti, per un dress code ad hoc (mai la dicitura in inglese, che significa "abbigliamento richiesto", è più appropriata!), quindi sbizzarritisci pure con parrucche stravaganti, RockyHorrorPictureShow style, catene dorate, strass e paillettes e ovviamente ben venga un attualissimo e perfetto Electro dress code! Quì puoi trovare spunti per il tuo E'n'R' look: www.clubtrailertrash.com - www.buggedout.net - www.myspace.com/thesupersuper - www.pigmag.com Tutti gli avventori delle RockboardNight potranno godere anche delle altre sonorità proposte nella serata, ognuna in una sala dedicata: _Rock 360°, Vintage e Hip-Hop (www.rockboard.it)_ --- La situazione che ha visto protagonista la Drum'n'Bass (www.massivenight.com), lo scorso anno di venerdì, viene spostata al sabato in concomitanza con le serate Re:Flex (pura elettronica - www.myspace.com/reflexnuclub) organizzate da Metempsicosi
    Computer Music & Project Studio

    Computer Music & Project Studio



    Jun 23 2009 11:48 AM

    Ciao Alesio
    grazie per l'amicizia e scusa per il ritardo.
    Ho difficoltà a visualizzare il tuo space sul Mac.
    Ho sentito i pezzi, rivedrei alcuni suoni e arrangiamenti. Con cosa produci?
    ciao
    Luca
    ---TARICK1---

    ---TARICK1---



    Jun 3 2009 3:32 PM

    Ciao
    vieni ad ascoltare i nostri nuovi remix di:
    Meg
    Subsonica
    Tre allegri allegri ragazzi morti
    Banshee
    Meganoidi
    etc
    etc
    etc
    su

    www.italiaremiscelata.com
    DRESS:CODE Discoteka

    DRESS:CODE ex Insomnia



    May 23 2009 8:33 AM

    ciao! oh, ma lo sai che noi siam partiti settimana scorsa con le RockboardNight estive al Gens di Pontedera?...mmmhhh, risponderai " e chi se ne frega!" o "già so!" o "ma dai? non lo sapevo!", va be a te la scelta, comunque ci troverai lì tutti i venerdì sino a metà settembre - - - INGRESSO OMAGGIO dalle 23.45 alle 00.30 - a seguire, con consumazione: 8euro Intero o 6euro con RockboardCard (scaricabile gratuitamente su www.rockboard.it) - - - - COME RAGGUNGERE IL GENS di Pontedera: FI-PI-LI Uscita Ponsacco, alla rotonda proseguire dritto in direzione Gello Pontedera, dopo 3 blocchi di semafori sulla sx trovi ingresso Area Gens, fai la stradina nei campi e arrivi al parcheggio custodito ----- INFO 3394606000 3481584045 www.rockboard.it – www.myspace.com/dresscode_disco.........Allora a presto! ------- poi se ti va dai un occhio all'Electro Eco Videoclip, dove partecipano Violet, Morgana, Marikey, ElectroBabe (la patty) e il nostro vecchio presidente onorario (max)....VOTALO su TALENT ONE di Italia 1....a questo link: http://www.talent1.mediaset.it/talentone/video/videotalent_25332.shtml - GRAZIE
    stonature

    Stonature Rec.



    May 21 2009 10:33 AM

    Salve ragazzi,
    avete bisogno di un furgone per il vostro tour?
    Per contatti 338.3103356 - stonature2000@yahoo.it
    Coast 2 Coast Mixtapes

    Coast 2 Coast Mixtapes



    Apr 8 2009 8:49 PM


    Check out our newest mixtape officially hosted by a Major Artist! Just click the cover to download all the Coast 2 Coast Mixtapes for free at www.coast2coastmixtapes.com!





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    Michele Magnani



    Jan 8 2009 5:33 PM




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    VIRTUAL live

    Lorenzo Mori



    Jan 7 2009 1:12 PM

    oooh! è uscito il disco dei LOWAVE!!! vai ad ascoltare i pezzi sul loro MySpace (http://www. myspace. com/lowavenet)!
    DRESS:CODE Discoteka

    DRESS:CODE ex Insomnia



    Jan 7 2009 12:06 PM

    Ciao! BUON ANNO!!!!! ------- grazie ad un importante accordo con uno sponsor possiamo ufficialmente rendere noto che per tutto il 2009, LOTTIAMO CONTRO IL CARO EURO!!! tutti i venerdì INGRESSO 10EURO - 8EURO con RockboardCard - DALLE 23 ALLE 23.30 INGRESSO OMAGGIO! ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- il programma delle serate lo trovi su www. dresscodeweb. com e su www. myspace. com/dresscode_disco ----- per tutto il 2009, LOTTIAMO CONTRO IL CARO EURO!!! INGRESSO 10EURO - 8EURO con RockboardCard - DALLE 23 ALLE 23.30 INGRESSO OMAGGIO! ---- su www. rockboard. it scarichi gratuitamente la RockboardCard, mostrala all'ingresso per ricevere la riduzione (valida solo il venerdì) ------- Se ti piace la musica elettronica non perderti le serate del sabato del Dress:Code targate Re:Flex, prodotte da Metempsicosi, con i migliori dj set e guest djs internazionali (www. myspace. com/reflexnuclub). Per vedere il programma a venire dei venerdì e sabato del Dress:Code, vai su www. dresscodeweb. com - hot line 3481584045 - 3394606000
    DRESS:CODE Discoteka

    DRESS:CODE ex Insomnia



    Dec 20 2008 11:14 AM

    le Feste di Natale passale al Dress:Code!! tante feste e tanti regali dal Dress......... IL 26 DICEMBRE scappa da tombola e panettoni, zie logorroiche e i cuginetti urlanti........Natal Dress con Ladies Night, si fa festa rockeggiando e le Donne entrano GRATIS tutta la notte!!! -------- il 2 gennaio INGRESSO OMAGGIO TUTTA LA NOTTE!!!!! ------- poi per tutto il 2009, LOTTIAMO CONTRO IL CARO EURO!!! INGRESSO 10EURO - 8EURO con RockboardCard - DALLE 23 ALLE 23.30 INGRESSO OMAGGIO! ----- dalle 23.00 LIVE con: 26 dicembre Tinker Bell (Punk Rock) – Livorno – www. myspace. com/tinkerbellhb -/- 02 gennaio  Diluve (Metal) – Bientina (PI) - www. myspace. com/diluve -/-
    ------------- A seguire 4 AREE con dj set: Vintage Area (dagli anni ’50 ai ’90): 26 dicembre Miss Clawdy e Gagliano dj -/- 02 gennaio Gagliano dj e Maina dj + MixClassicMovies -/-
 + tutti i venerdì MixClassicMovies --------- Rock 360°: 26 dicembre Marta (RockPlanetProduction – Sick Girl) e Pippo dj -/- 02 gennaio  Lady Violet (RockPlanetProduction – ZOE Milano) + ScaVenGer (RBN) + 1000Kms dj (RBN) -/-
 + RockVJset con 500 Miles ------- Jumparty Area (Hip-Hop /R’n’B/Funky): 26 dicembre Dj Evolution -/- 02 gennaio  S-UP -/-
 ---- Drum’n’Bass Area: All Fridays the Big Nu Combo Crew + KVR visuals ----- il 2 gennaio INGRESSO OMAGGIO TUTTA LA NOTTE!!!!! per tutto il 2009, LOTTIAMO CONTRO IL CARO EURO!!! INGRESSO 10EURO - 8EURO con RockboardCard - DALLE 23 ALLE 23.30 INGRESSO OMAGGIO! ---- su www. rockboard. it scarichi gratuitamente la RockboardCard, mostrala all'ingresso per ricevere la riduzione (valida solo il venerdì) ------- Se ti piace la musica elettronica non perderti le serate del sabato del Dress:Code targate Re:Flex, prodotte da Metempsicosi, con i migliori dj set e guest djs internazionali (www. myspace. com/reflexnuclub). Per vedere il programma a venire dei venerdì e sabato del Dress:Code, vai su www. dresscodeweb. com - hot line 3481584045 - 3394606000
    DRESS:CODE Discoteka

    DRESS:CODE ex Insomnia



    Nov 13 2008 4:01 PM

    CIAO - come va? di seguito il PROGRAMMA fino a metà novembre della RockboardNight, il venerdì del Dress, per aggiornamenti futuri dai un occhio al sito www. dresscodeweb. com, dove trovi anche le news sul sabato e special events: ----- dalle 23.00 LIVE con: 14 novembre Barbed Wire (Trash Metal) – Pisa – www. myspace. com/myspacebarbedwire
21 novembre Temple Rents (Emo – Punk)– Genova (GE) – www. myspace. com/templerents 28 novembre Disowned (Nu Rock) – Pisa (PI) – www. myspace. com/disownedband ----- a seguire 4aree con DJ SET ----- Vintage Area (dagli anni ’50 ai ’90): 14 novembre Miss Clawdy e Gagliano dj -/- 21 novembre “Oldies but Goodies” by Miss Clawdy & Maina dj -/- 28 novembre Miss Clawdy e Gagliano + tutti i venerdì MixClassicMovies ----- Rock 360° Area: 14 novembre Stella dj (RockPlanetProduction – Rock’n’Roll Milano) e Pippo dj -/- 21 novembre Garavelli (ROCK FM) e Claudia (ROCK FM) -/-
28 novembre Morgana (RockPlanetProduction – Bambole di Pezza) e Pippo dj + tutti i venerdì RockVJset con 500 Miles ---- JumParty (hip-hop/funky/r&b): 14 novembre dj Evolution -/- 21 novembre S-UP -/- 28 novembre Dj Evolution ---- Drum’n’Bass Area: All Fridays the Big Nu Combo Crew + KVR visuals
----- INGRESSO 12EURO - 10EURO con RockboardCard - DALLE 23 ALLE 23.30 INGRESSO OMAGGIO! ---- su www. rockboard. it scarichi gratuitamente la RockboardCard, mostrala all'ingresso per ricevere la riduzione (valida solo il venerdì) ------- Se ti piace la musica elettronica non perderti le serate del sabato del Dress:Code targate Re:Flex, prodotte da Metempsicosi, con i migliori dj set e guest djs internazionali (www. myspace. com/reflexnuclub). Per vedere il programma a venire dei venerdì e sabato del Dress:Code, vai su www. dresscodeweb. com - hot line 3481584045 - 3394606000
    Vincenzo Favale

    Vincenzo Favale



    Oct 27 2008 3:25 AM

    Lukenji San

    Lukenji San



    Oct 27 2008 2:14 AM

    ELENOIRE Music Group

    ELENOIRE Music Group



    Oct 22 2008 8:23 AM









    M.E.N. Mike Er Nonno®

    Michele Magnani



    Oct 10 2008 11:04 PM




    se vuoi essere dei nostri in
    Piazza Duomo a Milano
    scrivi una mail o aggiungi ad msn:
    nonnovit@hotmail.com

    all'R&Baggiooooo!!!!!
    Max Paparella

    Max Paparella



    Sep 26 2008 2:41 PM

    Thank you very much for the friendship !!!

     Photobucket


    The album "Stack 'em high" is out now


    You can buy your copy here :




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    Listen to all songs free on this player . . .





    M.E.N. Mike Er Nonno®

    Michele Magnani



    Sep 3 2008 9:37 PM


    click the image to vote

    click the image to buy
    M.E.N. Mike Er Nonno®

    Michele Magnani



    Aug 27 2008 3:39 PM


    click the image to enter
    DRESS:CODE Discoteka

    DRESS:CODE ex Insomnia



    Aug 20 2008 2:49 PM

    Ciao! Grazie dell'add. e non ti preoccupare, noi gli amici li trattiamo bene! Ciao ------- La Banda del Dress:Code -------- NB ricorda che il Dress, inaugura le serate del venerdì RockboardNight il 19 settembre e quelle del sabato Reflex il 4 ottobre -------- Un'anteprIma: quest'anno il venerdì ci saranno le mitiche aree Rock 360°, Hip-hop e Vintage, nonchè il ritorno della DRUM N BASS!!! ------- Faremo il possibile per tenere informati tutti i nostri amici sulle news, ma tu, se vuoi, ogni tanto dai un occhio al nostro space, grazie. CHE SARA' AL 100% dai primi di settembre.
    7Th FLOOR

    7Th FLOOR



    Jul 12 2008 9:46 AM

    HEY YEAHHHHHHH!!!! SLAVES OF THE SYSTEM

    http://www. youtube. com/watch?v=JgSc-ykgplI
    streamfest

    streamfest



    Jun 17 2008 9:55 AM

    Fluorescent Grey

    Fluorescent Grey



    May 10 2008 8:04 AM



    listen to Anon Day's music here
    listen to Kush Arora's music here
    listen to Fluorescent Grey's music here
    ---TARICK1---

    ---TARICK1---



    Apr 16 2008 4:35 PM

    Ciao amici !
    Guardate il mio video su www. allmusic. tv
    e su www. repubblica.
    it XL, spero vi piaccia!
    *IL VALENTE* (!)

    *IL VALENTE* (!)



    Apr 14 2008 7:40 PM

    proprio una bella mossa si...
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